Expedited Newborn HIV-1 DNA PCR Testing Information

The 24/7 Illinois Perinatal HIV Hotline offers expedited HIV-1 DNA PCR tests for calls to the Hotline in which an urgent test result is needed for pediatric treatment decision making. Specimens are processed at the Special Infectious Disease (SID) Laboratory of the Ann & Robert H. Lurie Children’s Hospital of Chicago.

If you require expedited testing, you must first call the 24/7 Illinois Perinatal HIV Hotline at 1-800-439-4079. All tests must be requested through the Hotline.
 

Specimen Collection and Requirements:

EDTA collections are preferred but ACD collections are acceptable. Samples collected by heel stick or venipuncture are acceptable. 1.0mL EDTA blood is optimal, 0.5mL is minimum. Specimen must be collected in a dedicated purple top vacutainer tube or pediatric microtainer, which is properly packaged and shipped overnight or via local courier under ambient conditions.

Tube must be labeled with a minimum of 2 unique patient identifiers (Ex. name, date of birth)
Specimen must be accompanied by a completed SID Laboratory HIV-1 DNA PCR Requisition.


Specimen Shipment:

Notify the 24/7 Illinois Perinatal HIV Hotline at 1-800-439-4079 when you’ve decided to send a specimen for expedited testing.

Notify the SID Lab of an impending shipment so that it can be tracked and received. The SID Lab can be reached at 312-227-6290 (phone), 312-227-7490 (fax). The SID Lab is open 7:30AM to 6:00PM Monday - Friday.
Send specimen overnight or via local courier under ambient conditions to the following address:

Special Infectious Disease (SID) Laboratory
Lurie Children’s Hospital of Chicago
2515 N. Clark Street
Deming 3012
Chicago, IL 60614
312-227-6290

Samples collected on Saturday or Sunday should be held for Monday shipment.


Testing Schedule:

Processing of HIV-1 DNA PCR specimens is done Monday – Friday with results within 72 hours.
Processing of HIV-1 DNA PCR specimens is not performed on weekends, including Saturdays and Sundays.


Reporting of Results:

HIV-1 DNA PCR test results will be reported via fax or page to the clinician requiring the result from the requesting hospital. Be certain to include this information on the lab requisition.
For questions, the SID Lab can be reached at 312-227-6290 (phone).
Please call the Hotline directly at 1-800-439-4079 if you have additional questions.


Fee Schedule:

Test Code Test Name Charge CPT Codes
IRPCB HIV-1 DNA PCR, Qualitative $180.00 87535, 89501